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Electron Microscopy and Histology Facility (EMH)

Center for Molecular and Cellular Bioengineering

The Electron Microscopy and Histology Facility provides expertise and technology to study the histology and ultrastructure of cell and tissue samples. The EM Facility offers access to modern sample preparation technology as well as to transmission and scanning electron microscopes (TEM and SEM), it also provides EM services, which are performed in collaboration with the reserach groups. The Histology Facility provides expertise and technology for the histological analysis of tissues, embryos and small animals. It offers access to sample preparation technology for paraffin or resin embedding, and to different types of microtomes for cryo- and room temperature sectioning. It also provides services such as embedding, sectioning, and staining (different histological stains, immunostaining). The facility constantly improves sample preparation methods and adapts them to new sample types. It also develops new sample preparation techniques especially for correlative and multimodal microscopy approaches (e.g. CLEM).

EM Services

  • Processing and embedding of cells and tissues into epoxy resins (Epon, Spurrs resin)

  • Progressive Lowering of Temperature embedding (PLT) in methacrylate resins for immunogold labeling

  • Embedding into gelatin/sucrose followed by plunge freezing for Tokuyasu cryo-sectioning and immunogold labeling

  • Preembedding immunogold labeling usin Nanogold and silver enhancement

  • High pressure freezing (HPF) and freeze substitution (FS)

  • Ultramicrotomy (room temperature and cryo): semithin sections for light microscopy and ultrathin sections for TEM

  • Negative staining
  • Immunogold labeling of resin or Tokuyasu cryo-sections (postembedding labeling)
  • Critical point drying and sputter coating for SEM

     

Imaging

  • TEM data acquisition and analysis (TEM)
  • SEM data acquisition and analysis (SEM)
  • Correlative light electron microscopy (CLEM)

Histology Services

  • Embedding of tissue samples into paraffin
  • Embedding of tissue samples into methacrylate resin (Technovit 7100)
  • Whole mount immunolabeling for subsequent resin embedding and serial sectioning
  • Sectioning of paraffin or resin embedded or of frozen samples
  • Histological staining of sections (hematoxylin-eosin (H.E.), Giemsa, Toluidine-blue/Borax, Masson-Goldner Weigert van Gieson, silver staining)
  • Immunohistochemistry using fluorescence or enzyme-based (e.g. HRP-DAB) detection systems

Team

Thomas Kurth © CMCB

Thomas Kurth

Facility Leader

Susanne Kretschmar © CMCB

Susanne Kretschmar

Technician

Susanne Weiche © CMCB

Susanne Weiche

Technician

Visitor's Address

Center for Regenerative Therapies Dresden (CRTD)
Fetscherstraße 105
01307 Dresden
Germany

Visitor's Address

Biotechnology Center (BIOTEC)
Tatzberg 47/49
01307 Dresden
Germany

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