Technology Development Studio (TDS)

Max Planck Institute of Molecular Cell Biology and Genetics

The major goal of the High-Throughput Technology Development Studio (TDS) is to provide expertise in assay development towards high-throughput applications and high content screening services (HCS). To this end the HT-TDS has state-of-the-art technologies for cell-based screening such as robotic liquid handling stations, plate readers and several automated microscopes. The services include developing complex automated image analysis pipelines and multi-parametric analysis of high content imaging applications.

Techniques

Automated confocal microscopy (spinning disk, LSM)
Automated widefield microscopy
Plate reading technologies: Fluorescence (emission / polarization / time resolved), Alpha ELISA, Luminescence, Absorbance
Automated liquid handling (contact and free dispense, acoustic dispensing, plate dispensing / washing / shaking)
Real time metabolism measurement
Electroporation

Specialities

Complete execution of high-throughput / high-content screening projects
Provision of individual processes performed by TDS-staff (transfection, automated liquid handling, cherry picking, dilution series)
Screenable model systems (cell lines, primary / differentiated cells, 2D / 3D cultures, whole organism)
Assay Development (design, optimization, validation)
Automated microscopy (live / fixed, confocal / widefield, 2D / 3D)
Image and statistical analysis (CellProfiler, FIJI, KNIME, R, Python, high-performance computing)
User Facility
Reagent libraries: genome-wide siRNA / crRNA, focussed / picking libraries, chemical / drug discovery libraries