Test-Teaching

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Light Microscopy

Principles of light microscopy

Peter Evennett Course

• Basics of Microscopy (2008)
• Principles of Light Microscopy
• Lens Aberrations

Master Course

Koehler illumination

• Köhler Illumination (2008)

Image Formation

• Diffraction
• video by Peter Evennett: Abbe's diffraction experiments

Contrast techniques for transmitted light microscopy

• Diffraction, Resolution and Contrasting Techniques (2008)
• Contrast

Differential Interference Contrast (DIC) microscopy

• Interference Contrast

Polarized light microscopy

• Polarised Light

Stereomicroscopy

• Stereomicroscopes

Fluorescence microscopy

• Fluorescence Microscopy
• Basics of fluorescence Microscopy
• Fluorescence Imaging (2008)

Companies resources

• Optical Filters - Essential Tools by Dr. Michael Sommerauer (AHF)
• Optical Filters - Specifying Filters by Dr. Michael Sommerauer (AHF)

Advanced Microscopy

• FRET by Mike Lorenz (2009)
• FLIM by Mike Lorenz (2009)
• How to do FRET by Mike Lorenz (2009)
• FLIM-FRET data examples by Mike Lorenz (2009)

• Confocal Microscopy
• Optical Sectioning Methods - pros and cons (LMF)
• Deconvolution and Optical Sectioning - pros and cons-11-2010‎

• CCD Imaging (2008)
• Digital Imaging (2008)

Videos at Vimeo (in HD) These show Abbe's diffraction experiments (Peter Evennett), cool SPIM 3D reconstructions, and the effects of the confocal pinhole etc.

Image Processing

IPF Image Processing Courses

• What you need to know about Image Analysis - updated Oct 2011
• Quantitative Imaging for Colocalization Analysis (updated Oct 2011)
• Introduction to Fiji and Macro Programming (updated Jul 2011)
• Quantitative Imaging and Image Processing/Analysis 10-2009 (IPF)
• Quantitative Imaging and Basic Image Processing (2008)

• Fiji (is just ImageJ - batteries included) Tutorials

General Image Processing reading material

• Image manipulation ethics - Tutorial - avoid twisted pixels, and the original article

DIPP course from the TDS of the MPI-CBG

• Presentations about multifactorial experimental design, automated microscopy and image analysis - updated Oct 2011

• Presentations about multifactorial experimental design, automated microscopy and image analysis - updated Mar 2012

Matlab

• Matlab Fundamentals - December 2010

See also the handouts of the Matlab for biologists course at the Light Microscopy Centre of the ETH Zürich and the materials of the Matlab programming course for Biophysicists at Universidad Autónoma de Madrid.

Courses

Peter Evennett Course

Twice a year the BioDIP team together with Peter Evennett organizes a basic course about microscopy.

Schedules/Content

• BasicsCoursePhDprog-PeterEvennett - schedule Oct. 2013

• BasicsCoursePhDprog-PeterEvennetApril2013

• BasicsCoursePhDprog-PeterEvennetOctober2012

• BasicsCoursePhDprog-PeterEvennetApril2012

• BasicsCoursePhDprog-PeterEvennetOct2011-observed timing and needed materials

• BasicsCoursePhDprog-PeterEvennetMay2011-observed_timing

• BasicsCoursePhDprog-PeterEvennetMay2011

• Peter Evennett PhD course schedule Apr 2010

• PhD course schedule 2009 nov

• Peter E course schedule 2009 oct

Impression from the Peter Evennett course 2007

• 

  course announcement

• 

  Peter Evennett and Humberto at the optical bench

• 

  room overview - 2nd floor galeria

• 

  Peter at his demo microscope

• 

  it's all about lenses

• 

  Jan

• 

  the optical bench

• 

  Peter at the condenser

• 

  demonstration of refractive index with a cup, a coin and water

• 

  Peter Evennett

• 

  Peter & Peter, setting up the demo microscope

• 

  Tim Cross, finally at the pipette

Other Resources

• Papers
• Links