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	* [[media:Microscopy_principles_guide-part-1.pdf|Basics of Microscopy (2008)]]		* [[media:Microscopy_principles_guide-part-1.pdf|Basics of Microscopy (2008)]]
−	== Koehler illumination ==	+	== Illumination ==
	'''Peter Evennett Course'''<br>		'''Peter Evennett Course'''<br>
	* [[media:Evennett_oct2008_01_principles-of-microscopy-part-2.pdf|Köhler Illumination]]		* [[media:Evennett_oct2008_01_principles-of-microscopy-part-2.pdf|Köhler Illumination]]

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Revision as of 16:48, 16 January 2014

delete gallery page and integrate it here

Contents 1 Light Microscopy 1.1 Principles of Light Microscopy 1.2 Illumination 1.3 Theory of Image Formation 1.4 Contrast Techniques for Transmitted Light Microscopy 1.4.1 Dark Field Microscopy 1.4.2 Phase Contrast Microscopy 1.4.3 Polarized Light microscopy 1.4.4 Differential Interference Contrast (DIC) microscopy 1.5 Stereomicroscopy 1.6 Fluorescence Microscopy 2 Advanced Microscopy 3 Image Processing 3.1 IPF Image Processing Courses 3.2 General Image Processing reading material 3.3 DIPP course from the TDS of the MPI-CBG 3.4 Matlab 4 Courses 4.1 Peter Evennett Course 4.1.1 Schedules/Content 4.1.2 Impression from the Peter Evennett course 2007 4.2 Master Course 5 Other Resources

Light Microscopy

Principles of Light Microscopy

Peter Evennett Course

• Principles of Light Microscopy
• Lens Aberrations

Master Course
BioDIP Course (2008)

• Basics of Microscopy (2008)

Illumination

Peter Evennett Course

• Köhler Illumination

Master Course
BioDIP Course (2008)

• Köhler Illumination (2008)
• How to set Köhler Illumination (2008)

Theory of Image Formation

Peter Evennett Course

• Diffraction
• video by Peter Evennett: Abbe's diffraction experiments

Master Course
BioDIP Course (2008)

• Diffraction

Contrast Techniques for Transmitted Light Microscopy

Peter Evennett Course

• Contrast

Master Course
BioDIP Course (2008)

• Contrasting Techniques

Dark Field Microscopy

Peter Evennett Course
Master Course
BioDIP Course (2008)

• Dark Field Microscopy

Phase Contrast Microscopy

Peter Evennett Course
Master Course
BioDIP Course (2008)

• Phase Contrast Microscopy

Polarized Light microscopy

Peter Evennett Course

• Polarised Light

Master Course
BioDIP Course (2008)

• Polarized Light Microscopy

Differential Interference Contrast (DIC) microscopy

Peter Evennett Course

• Interference Contrast

Master Course
BioDIP Course (2008)

• DIC Microscopy

Stereomicroscopy

Peter Evennett Course

• Stereomicroscopes

Master Course
BioDIP Course (2008)

Fluorescence Microscopy

Peter Evennett Course

• Fluorescence Microscopy
• Basics of fluorescence Microscopy

Master Course
BioDIP Course (2008)

• Fluorescence Imaging (2008)

External Resources

• Optical Filters - Essential Tools by Dr. Michael Sommerauer (AHF)
• Optical Filters - Specifying Filters by Dr. Michael Sommerauer (AHF)

Advanced Microscopy

Peter Evennett Course

• Confocal Microscopy
• Optical Sectioning Methods - pros and cons (LMF)
• Deconvolution and Optical Sectioning - pros and cons-11-2010‎

Videos at Vimeo (in HD) These show Abbe's diffraction experiments (Peter Evennett), cool SPIM 3D reconstructions, and the effects of the confocal pinhole etc.
Master Course
BioDIP Course (2008)

• CCD Imaging (2008)
• Digital Imaging (2008)
• FRET by Mike Lorenz (2009)
• FLIM by Mike Lorenz (2009)
• How to do FRET by Mike Lorenz (2009)
• FLIM-FRET data examples by Mike Lorenz (2009)

Image Processing

IPF Image Processing Courses

• What you need to know about Image Analysis - updated Oct 2011
• Quantitative Imaging for Colocalization Analysis (updated Oct 2011)
• Introduction to Fiji and Macro Programming (updated Jul 2011)
• Quantitative Imaging and Image Processing/Analysis 10-2009 (IPF)
• Quantitative Imaging and Basic Image Processing (2008)

• Fiji (is just ImageJ - batteries included) Tutorials

General Image Processing reading material

• Image manipulation ethics - Tutorial - avoid twisted pixels, and the original article

DIPP course from the TDS of the MPI-CBG

• Presentations about multifactorial experimental design, automated microscopy and image analysis - updated Oct 2011

• Presentations about multifactorial experimental design, automated microscopy and image analysis - updated Mar 2012

Matlab

• Matlab Fundamentals - December 2010

See also the handouts of the Matlab for biologists course at the Light Microscopy Centre of the ETH Zürich and the materials of the Matlab programming course for Biophysicists at Universidad Autónoma de Madrid.

Courses

Peter Evennett Course

Twice a year the BioDIP team together with Peter Evennett organizes a basic course about microscopy.

Schedules/Content

• BasicsCoursePhDprog-PeterEvennett - schedule Oct. 2013

• BasicsCoursePhDprog-PeterEvennetApril2013

• BasicsCoursePhDprog-PeterEvennetOctober2012

• BasicsCoursePhDprog-PeterEvennetApril2012

• BasicsCoursePhDprog-PeterEvennetOct2011-observed timing and needed materials

• BasicsCoursePhDprog-PeterEvennetMay2011-observed_timing

• BasicsCoursePhDprog-PeterEvennetMay2011

• Peter Evennett PhD course schedule Apr 2010

• PhD course schedule 2009 nov

• Peter E course schedule 2009 oct

Impression from the Peter Evennett course 2007

• 

  course announcement

• 

  Peter Evennett and Humberto at the optical bench

• 

  room overview - 2nd floor galeria

• 

  Peter at his demo microscope

• 

  it's all about lenses

• 

  Jan

• 

  the optical bench

• 

  Peter at the condenser

• 

  demonstration of refractive index with a cup, a coin and water

• 

  Peter Evennett

• 

  Peter & Peter, setting up the demo microscope

• 

  Tim Cross, finally at the pipette

Master Course

Other Resources

• Papers
• Links