Test-Teaching
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== Contrast Techniques for Transmitted Light Microscopy== | == Contrast Techniques for Transmitted Light Microscopy== | ||
| − | '''Peter Evennett Course'''<br> | + | Different techniques exist to enhance the contrast in your sample. In this section Contrasting Techniques are described. |
| + | |||
| + | |||
| + | '''Materials from the Peter Evennett Course'''<br> | ||
* [[media:Evennett oct2008 04 contrast-1.pdf|Contrast]] | * [[media:Evennett oct2008 04 contrast-1.pdf|Contrast]] | ||
| − | '''Master Course'''<br> | + | '''Materials from the Master Course'''<br> |
| − | '''BioDIP Course (2008)'''<br> | + | '''Materials from the BioDIP Course (2008)'''<br> |
* [[media:Contrasting_techniques-2.pdf|Contrasting Techniques]] | * [[media:Contrasting_techniques-2.pdf|Contrasting Techniques]] | ||
=== Dark Field Microscopy=== | === Dark Field Microscopy=== | ||
| − | '''Peter Evennett Course'''<br> | + | '''Materials from the Peter Evennett Course'''<br> |
| − | * [[media:Evennett oct2008 04 contrast-2-dark-field.pdf|Dark Field]] | + | * [[media:Evennett oct2008 04 contrast-2-dark-field.pdf|Dark Field Microscopy]] |
| − | '''Master Course'''<br> | + | '''Materials from the Master Course'''<br> |
| − | '''BioDIP Course (2008)'''<br> | + | '''Materials from the BioDIP Course (2008)'''<br> |
* [[media:Contrasting_techniques-3-dark-field.pdf|Dark Field Microscopy]] | * [[media:Contrasting_techniques-3-dark-field.pdf|Dark Field Microscopy]] | ||
=== Phase Contrast Microscopy=== | === Phase Contrast Microscopy=== | ||
| − | '''Peter Evennett Course'''<br> | + | '''Materials from the Peter Evennett Course'''<br> |
| − | * [[media:Evennett oct2008 04 contrast-3-phase-contrast.pdf|Phase Contrast]] | + | * [[media:Evennett oct2008 04 contrast-3-phase-contrast.pdf|Phase Contrast Microscopy]] |
| − | '''Master Course'''<br> | + | '''Materials from the Master Course'''<br> |
| − | '''BioDIP Course (2008)'''<br> | + | '''Materials from the BioDIP Course (2008)'''<br> |
* [[media:Contrasting_techniques-4-phase contrast.pdf|Phase Contrast Microscopy]] | * [[media:Contrasting_techniques-4-phase contrast.pdf|Phase Contrast Microscopy]] | ||
=== Polarized Light microscopy=== | === Polarized Light microscopy=== | ||
| − | '''Peter Evennett Course'''<br> | + | '''Materials from the Peter Evennett Course'''<br> |
| − | * [[media:Evennett_oct2008_05_polarised-light.pdf| | + | * [[media:Evennett_oct2008_05_polarised-light.pdf|Polarized Light Microscopy]] |
| − | '''Master Course'''<br> | + | '''Materials from the Master Course'''<br> |
| − | '''BioDIP Course (2008)'''<br> | + | '''Materials from the BioDIP Course (2008)'''<br> |
* [[media:Contrasting_techniques-5-polarized light.pdf|Polarized Light Microscopy]] | * [[media:Contrasting_techniques-5-polarized light.pdf|Polarized Light Microscopy]] | ||
=== Differential Interference Contrast (DIC) microscopy=== | === Differential Interference Contrast (DIC) microscopy=== | ||
| − | '''Peter Evennett Course'''<br> | + | '''Materials from the Peter Evennett Course'''<br> |
| − | * [[media:Evennett_oct2008_06_interference-contrast.pdf| | + | * [[media:Evennett_oct2008_06_interference-contrast.pdf|DIC Microscopy]] |
| − | '''Master Course'''<br> | + | '''Materials from the Master Course'''<br> |
| − | '''BioDIP Course (2008)'''<br> | + | '''Materials from the BioDIP Course (2008)'''<br> |
* [[media:Contrasting_techniques-6-DIC.pdf|DIC Microscopy]] | * [[media:Contrasting_techniques-6-DIC.pdf|DIC Microscopy]] | ||
Revision as of 17:31, 16 January 2014
delete gallery page and integrate it here
Contents |
Light Microscopy
Principles of Light Microscopy
The materials presented here contain basic information about the physics of light, the optics of lenses and the components of a microscope.
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Illumination
This section contains information about proper illumination of your sample in the microscope and details on one method termed "Köhler Illumination".
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Theory of Image Formation
This section describes how the microscopical image is formed by diffraction and what consequences (like resolution) arise from that.
Materials from the Peter Evennett Course
A video by Peter Evennett showing Abbes diffraction experiments can be found here or on Vimeo (in HD).
Materials from the Master Course
Materials from the BioDIP Course (2008)
Contrast Techniques for Transmitted Light Microscopy
Different techniques exist to enhance the contrast in your sample. In this section Contrasting Techniques are described.
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Dark Field Microscopy
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Phase Contrast Microscopy
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Polarized Light microscopy
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Differential Interference Contrast (DIC) microscopy
Materials from the Peter Evennett Course
Materials from the Master Course
Materials from the BioDIP Course (2008)
Stereomicroscopy
Peter Evennett Course
Master Course
BioDIP Course (2008)
Fluorescence Microscopy
Peter Evennett Course
Master Course
BioDIP Course (2008)
External Resources
- Optical Filters - Essential Tools by Dr. Michael Sommerauer (AHF)
- Optical Filters - Specifying Filters by Dr. Michael Sommerauer (AHF)
Advanced Microscopy
Peter Evennett Course
- Confocal Microscopy
- Optical Sectioning Methods - pros and cons (LMF)
- Deconvolution and Optical Sectioning - pros and cons-11-2010
Videos at Vimeo (in HD)
These show Abbe's diffraction experiments (Peter Evennett), cool SPIM 3D reconstructions, and the effects of the confocal pinhole etc.
Master Course
BioDIP Course (2008)
- CCD Imaging (2008)
- Digital Imaging (2008)
- FRET by Mike Lorenz (2009)
- FLIM by Mike Lorenz (2009)
- How to do FRET by Mike Lorenz (2009)
- FLIM-FRET data examples by Mike Lorenz (2009)
Image Processing
IPF Image Processing Courses
- What you need to know about Image Analysis - updated Oct 2011
- Quantitative Imaging for Colocalization Analysis (updated Oct 2011)
- Introduction to Fiji and Macro Programming (updated Jul 2011)
- Quantitative Imaging and Image Processing/Analysis 10-2009 (IPF)
- Quantitative Imaging and Basic Image Processing (2008)
General Image Processing reading material
DIPP course from the TDS of the MPI-CBG
Matlab
See also the handouts of the Matlab for biologists course at the Light Microscopy Centre of the ETH Zürich and the materials of the Matlab programming course for Biophysicists at Universidad Autónoma de Madrid.
Courses
Peter Evennett Course
Twice a year the BioDIP team together with Peter Evennett organizes a basic course about microscopy.
Schedules/Content
Impression from the Peter Evennett course 2007
course announcement
Peter Evennett and Humberto at the optical bench
room overview - 2nd floor galeria
Peter at his demo microscope
it's all about lenses
Jan
the optical bench
Peter at the condenser
demonstration of refractive index with a cup, a coin and water
Peter Evennett
Peter & Peter, setting up the demo microscope
Tim Cross, finally at the pipette
