Test-Teaching

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= Light Microscopy =
 
= Light Microscopy =
 +
== Principles of Light Microscopy ==
 +
The materials presented here contain basic information about the physics of light, the optics of lenses and the components of a microscope.
  
 +
'''Materials from the Peter Evennett Course'''<br>
 +
* [[media:Evennett_oct2008_01_principles-of-microscopy-part-1.pdf|Principles of Light Microscopy]]
 +
* [[media:Evennett_oct2008_02_lens-aberrations.pdf|Lens Aberrations ]]
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:Microscopy_principles_guide-part-1.pdf|Basics of Microscopy (2008)]]
  
−
* [[media:Stereomicroscopes_notes.pdf|Stereomicroscopes]]
+
== Illumination ==
 +
This section contains information about proper illumination of your sample in the microscope and details on one method termed "Köhler Illumination".
  
−
== Principles of light microscopy ==
+
'''Materials from the Peter Evennett Course'''<br>
−
* [[media:Microscopy_principles_guide.pdf|Basics of Microscopy (2008)]]
+
* [[media:Evennett_oct2008_01_principles-of-microscopy-part-2.pdf|Microscope Illumination]]
−
* [[media:Evennett_oct2008_01_principles-of-microscopy.pdf|Principles of Light Microscopy]]
+
'''Materials from the BioDIP Course (2008)'''<br>
−
* [[media:Evennett_oct2008_02_lens-aberrations.pdf|Lens Aberrations ]]
+
* [[media:Microscopy_principles_guide-part-2.pdf|Microscope Illumination (2008)]]
−
== Koehler illumination ==
+
* [[media:Ibarra_how-to-set-koehler-illumination.pdf|How to set Köhler Illumination (2008)]]
−
* [[media:Ibarra_how-to-set-koehler-illumination.pdf|Köhler Illumination (2008)]]
+
 
−
== Image Formation ==
+
== Theory of Image Formation ==
−
* [[media:Evennett_oct2008_03_diffraction.pdf|Diffraction ]]
+
 
−
* [[Peter_Evennett_-_Abbe_Diffraction_Experiments|video by Peter Evennett: Abbe's diffraction experiments]]
+
This section describes how the microscopical image is formed by diffraction and what consequences (like resolution) arise from that.
−
== Contrast techniques for transmitted light microscopy==
+
 
−
* [[media:Contrasting_techniques.pdf|Diffraction, Resolution and Contrasting Techniques (2008)]]
+
'''Materials from the Peter Evennett Course'''<br>
−
* [[media:Evennett_oct2008_04_contrast.pdf|Contrast]]
+
* [[media:Evennett_oct2008_03_diffraction.pdf|Diffraction and the Microscope Image]]
−
=== Dark field microscopy===
+
 
−
=== Phase contrast microscopy===
+
A video by Peter Evennett showing Abbes diffraction experiments can be found [[Peter_Evennett_-_Abbe_Diffraction_Experiments|here]] or on [http://vimeo.com/user795818/videos/sort:oldest Vimeo (in HD)].
 +
 
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:Contrasting techniques-1.pdf|Diffraction ]]
 +
 
 +
== Contrast Techniques for Transmitted Light Microscopy==
 +
Different techniques exist to enhance the contrast in your sample. In this section these contrasting techniques are described.
 +
 +
'''Materials from the Peter Evennett Course'''<br>
 +
* [[media:Evennett oct2008 04 contrast-1.pdf|Contrast]]
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:Contrasting_techniques-2.pdf|Contrasting Techniques]]
 +
=== Dark Field Microscopy===
 +
'''Materials from the Peter Evennett Course'''<br>
 +
* [[media:Evennett oct2008 04 contrast-2-dark-field.pdf|Dark Field Microscopy]]
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:Contrasting_techniques-3-dark-field.pdf|Dark Field Microscopy]]
 +
=== Phase Contrast Microscopy===
 +
'''Materials from the Peter Evennett Course'''<br>
 +
* [[media:Evennett oct2008 04 contrast-3-phase-contrast.pdf|Phase Contrast Microscopy]]
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:Contrasting_techniques-4-phase contrast.pdf|Phase Contrast Microscopy]]
 +
=== Polarized Light microscopy===
 +
'''Materials from the Peter Evennett Course'''<br>
 +
* [[media:Evennett_oct2008_05_polarised-light.pdf|Polarized Light Microscopy]]
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:Contrasting_techniques-5-polarized light.pdf|Polarized Light Microscopy]]
 
=== Differential Interference Contrast (DIC) microscopy===
 
=== Differential Interference Contrast (DIC) microscopy===
−
* [[media:Evennett_oct2008_06_interference-contrast.pdf|Interference Contrast]]
+
'''Materials from the Peter Evennett Course'''<br>
−
=== Polarized light microscopy===
+
* [[media:Evennett_oct2008_06_interference-contrast.pdf|DIC Microscopy]]
−
* [[media:Evennett_oct2008_05_polarised-light.pdf|Polarised Light]]
+
'''Materials from the BioDIP Course (2008)'''<br>
−
== Fluorescence microscopy ==
+
* [[media:Contrasting_techniques-6-DIC.pdf|DIC Microscopy]]
 +
 
 +
== Stereomicroscopy ==
 +
'''Peter Evennett Course'''<br>
 +
* [[media:Stereomicroscopes_notes.pdf|Stereomicroscopes]]
 +
'''BioDIP Course (2008)'''<br>
 +
 
 +
== Fluorescence Microscopy ==
 +
'''Peter Evennett Course'''<br>
 
* [[media:Fluorescence_notes.pdf|Fluorescence Microscopy]]
 
* [[media:Fluorescence_notes.pdf|Fluorescence Microscopy]]
 
* [[media:Fluorescence_basics.pdf| Basics of fluorescence Microscopy]]
 
* [[media:Fluorescence_basics.pdf| Basics of fluorescence Microscopy]]
 +
'''BioDIP Course (2008)'''<br>
 
* [[media:Fluorescence_Imaging.pdf|Fluorescence Imaging (2008)]]
 
* [[media:Fluorescence_Imaging.pdf|Fluorescence Imaging (2008)]]
−
 
+
'''External Resources'''<br>
−
Companies resources
+
−
 
+
 
* [[media:OpticalFilters_EssentialTools_08-12-09.pdf| Optical Filters - Essential Tools by Dr. Michael Sommerauer (AHF)]]
 
* [[media:OpticalFilters_EssentialTools_08-12-09.pdf| Optical Filters - Essential Tools by Dr. Michael Sommerauer (AHF)]]
 
* [[media:OpticalFilters_SpecifyingFilters_08-12-09.pdf| Optical Filters - Specifying Filters by Dr. Michael Sommerauer (AHF)]]
 
* [[media:OpticalFilters_SpecifyingFilters_08-12-09.pdf| Optical Filters - Specifying Filters by Dr. Michael Sommerauer (AHF)]]
 +
 +
= Advanced Microscopy =
 +
'''Materials from the Peter Evennett Course'''<br>
 +
* [[media:Confocal_notes.pdf|Confocal Microscopy]]
 +
* [[media:Optical_Sectioning_ProsAndCons10-2009.pdf|Optical Sectioning Methods - pros and cons (LMF)]]
 +
* [[media:DeconvolutionAndOpticalSectioning-prosandcons-11-2010.pdf|Deconvolution and Optical Sectioning - pros and cons-11-2010‎]]
 +
At [http://vimeo.com/user795818/videos/sort:oldest Vimeo(in HD)] we deposited some videos showing cool SPIM 3D reconstructions and the effects of the confocal pinhole etc.<br>
 +
 +
'''Materials from the BioDIP Course (2008)'''<br>
 +
* [[media:CCD_intro_2008_oct.pdf|CCD Imaging (2008)]]
 +
* [[media:Digital_imaging_oct_2008.pdf|Digital Imaging (2008)]]
 +
* [[media:FRET.pdf|FRET by Mike Lorenz (2009)]]
 +
* [[media:FLIM.pdf|FLIM by Mike Lorenz (2009)]]
 +
* [[media:How_to_do_FRET.pdf|How to do FRET by Mike Lorenz (2009)]]
 +
* [[media:FLIM-FRET_data_examples.pdf|FLIM-FRET data examples by Mike Lorenz (2009)]]
  
 
= Image Processing =
 
= Image Processing =
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See also the handouts of the [http://www.lmc.ethz.ch/Teaching/matlab_course Matlab for biologists] course at the Light Microscopy Centre of the ETH Z&uuml;rich and the [http://www.neural-circuits.org/teaching/programming-with-matlab materials] of the Matlab programming course for Biophysicists at Universidad Autónoma de Madrid.
 
See also the handouts of the [http://www.lmc.ethz.ch/Teaching/matlab_course Matlab for biologists] course at the Light Microscopy Centre of the ETH Z&uuml;rich and the [http://www.neural-circuits.org/teaching/programming-with-matlab materials] of the Matlab programming course for Biophysicists at Universidad Autónoma de Madrid.
−
 
−
= Advanced Microscopy =
 
−
* [[media:FRET.pdf|FRET by Mike Lorenz (2009)]]
 
−
* [[media:FLIM.pdf|FLIM by Mike Lorenz (2009)]]
 
−
* [[media:How_to_do_FRET.pdf|How to do FRET by Mike Lorenz (2009)]]
 
−
* [[media:FLIM-FRET_data_examples.pdf|FLIM-FRET data examples by Mike Lorenz (2009)]]
 
−
 
−
* [[media:Confocal_notes.pdf|Confocal Microscopy]]
 
−
* [[media:Optical_Sectioning_ProsAndCons10-2009.pdf|Optical Sectioning Methods - pros and cons (LMF)]]
 
−
* [[media:DeconvolutionAndOpticalSectioning-prosandcons-11-2010.pdf|Deconvolution and Optical Sectioning - pros and cons-11-2010‎]]
 
−
 
−
 
−
* [[media:CCD_intro_2008_oct.pdf|CCD Imaging (2008)]]
 
−
* [[media:Digital_imaging_oct_2008.pdf|Digital Imaging (2008)]]
 
−
 
−
[http://vimeo.com/user795818/videos/sort:oldest Videos at Vimeo (in HD)]
 
−
These show Abbe's diffraction experiments (Peter Evennett), cool SPIM 3D reconstructions, and the effects of the confocal pinhole etc.
 
  
 
= Courses =
 
= Courses =

Latest revision as of 17:37, 16 January 2014

delete gallery page and integrate it here

Contents

[edit] Light Microscopy

[edit] Principles of Light Microscopy

The materials presented here contain basic information about the physics of light, the optics of lenses and the components of a microscope.

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Illumination

This section contains information about proper illumination of your sample in the microscope and details on one method termed "Köhler Illumination".

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Theory of Image Formation

This section describes how the microscopical image is formed by diffraction and what consequences (like resolution) arise from that.

Materials from the Peter Evennett Course

A video by Peter Evennett showing Abbes diffraction experiments can be found here or on Vimeo (in HD).

Materials from the BioDIP Course (2008)

[edit] Contrast Techniques for Transmitted Light Microscopy

Different techniques exist to enhance the contrast in your sample. In this section these contrasting techniques are described.

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Dark Field Microscopy

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Phase Contrast Microscopy

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Polarized Light microscopy

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Differential Interference Contrast (DIC) microscopy

Materials from the Peter Evennett Course

Materials from the BioDIP Course (2008)

[edit] Stereomicroscopy

Peter Evennett Course

BioDIP Course (2008)

[edit] Fluorescence Microscopy

Peter Evennett Course

BioDIP Course (2008)

External Resources

[edit] Advanced Microscopy

Materials from the Peter Evennett Course

At Vimeo(in HD) we deposited some videos showing cool SPIM 3D reconstructions and the effects of the confocal pinhole etc.

Materials from the BioDIP Course (2008)

[edit] Image Processing

[edit] IPF Image Processing Courses

[edit] General Image Processing reading material

[edit] DIPP course from the TDS of the MPI-CBG

[edit] Matlab

See also the handouts of the Matlab for biologists course at the Light Microscopy Centre of the ETH Zürich and the materials of the Matlab programming course for Biophysicists at Universidad Autónoma de Madrid.

[edit] Courses

[edit] Peter Evennett Course

Twice a year the BioDIP team together with Peter Evennett organizes a basic course about microscopy.

[edit] Schedules/Content

[edit] Impression from the Peter Evennett course 2007

  • course announcement

  • Peter Evennett and Humberto at the optical bench

  • room overview - 2nd floor galeria

  • Peter at his demo microscope

  • it's all about lenses

  • Jan

  • the optical bench

  • Peter at the condenser

  • demonstration of refractive index with a cup, a coin and water

  • Peter Evennett

  • Peter & Peter, setting up the demo microscope

  • Tim Cross, finally at the pipette

[edit] Master Course

[edit] Other Resources

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